Identifying Pigmentation-Related Genes in Ophiostoma piceae Using Agrobacterium-Mediated Integration
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چکیده
منابع مشابه
Phylogeny and taxonomy of the Ophiostoma piceae complex and the Dutch elm disease fungi
The Ophiostoma piceae complex forms a monophyletic group of insect-dispersed pyrenomycetes with synnemata (Pesotum) and micronematous (Sporothrix) synanamorphs. Other species of Ophiostoma outside of the 0. piceae complex that form synnemata lack the Sporothrix state. The nine recognized species within the 0. piceae complex are delimited by synnema morphology, growth rate at 32 C, mating reacti...
متن کاملRecombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastoris
BACKGROUND The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity towards p-nitrophenol, glycerol and sterol esters. Its hydrolytic activity on natural mixtures of triglycerides and sterol esters has been proposed for pitch biocontrol in paper industry since these compounds produce important economic losses during paper pulp manufacture. RESULTS Recently, this en...
متن کاملRapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR.
A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhab...
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The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, and the hydrolytic activity of the recombinant protein (OPE*) turned out to be...
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Fungi of the Metarhizium genus are a very versatile model for understanding pathogenicity in insects and their symbiotic relationship with plants. To establish a co-transformation system for the transformation of multiple M. robertsii genes using Agrobacterium tumefaciens, we evaluated whether the antibiotic nourseothricin has the same marker selection efficiency as phosphinothricin using separ...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2007
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto-97-9-1040